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KMID : 0370220230670010051
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Yakhak Hoeji
2023 Volume.67 No. 1 p.51 ~ p.57
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Effect of Indirubin on Morphology Changes and mRNA Expression of Cytokines in LPS-induced Raw 264.7
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Hwang Ye-Jin
Kang Jae-Seon
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Abstract
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This study was conducted to investigate the regulatory effect of Indirubin on LPS-induced inflammation inmacrophage. The morphologic changes were shown as widened cytoplasm or biased nucleus from the center of the cellwhen LPS activated macrophage. The changes in inflammation activity were also estimated with mRNA levels ofcytokines under time elapse at 2, 4, 8, 12, and 24 hours after the 10 ng/mL LPS treatment. By measuring those proinflammatory(TNF-α, IL-1β) and anti-inflammatory (IL-10) cytokines at each time set, the relationship betweenmorphologic change and inflammation activity was compared. The highest pro-inflammatory mRNA expression wasobserved at 2 hours after the LPS treatment, and this was inhibited by those Indirubin standard (Indirubin) and Chungdaeextract (CDEX). The mRNA expression level in LPS treated cell was markedly reduced at 8 hours as well as the othergroups. However, IL-10, the anti-inflammatory cytokines was not affected by those treatments. The results suggested thatthe level of inflammation related changes as time elapsed. The current observation that both Indirubin and CDEX are ableto inhibit LPS-induced inflammation by reducing the production of inflammatory cytokines. Moreover, the currentobservation suggested that estimating anti-inflammatory effects should include time-dependent measurements as biomarkers.
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KEYWORD
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Chungdae, Indirubin, Inflammation, Macrophage, Morphology, mRNA, Time-dependent manner
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